anti synapsin1 (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc
anti synapsin1
Anti Synapsin1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 379 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+synapsin1/Synapsin-1+XP+Rabbit+mAb/pm41106508-82-48-51
Average 96 stars, based on 379 article reviews
Anti Synapsin1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 379 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+synapsin1/Synapsin-1+XP+Rabbit+mAb/pm41106508-82-48-51
Average 96 stars, based on 379 article reviews
anti synapsin1 - by Bioz Stars,
2026-11
96/100 stars
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Incubation:Article Title: Human iPSC-Derived Cortical Neurons Display Homeostatic Plasticity Article Snippet: Fixed cells were then permeabilized using a PBS solution containing 0.2% Triton X-100 (Merck Life Science) for 15 min and blocked with the same solution supplemented with 5% goat serum (Merck Life Science) for 20 min at room temperature. .. Cells were then incubated with primary antibodies overnight at 4 °C using the following dilutions: mouse anti-PAX6 (sc81649 Santa Cruz Biotechnology, 1:50), chicken anti-MAP2 (ab5392 Abcam, 1:2000), rabbit anti-β-TUBULIN III (TUJ1) (T2200 Merck Life Science, 1:2000), mouse anti-GFAP (MAB360 Merck Life Science, 1:500), rabbit anti-PSD95 (3450 Cell Signaling, Danvers, MA; USA, 1:250), mouse anti-VGLUT1 (135303 Synaptic Systems, Göttingen, Germany, 1:250), Article Title: Human iPSC-Derived Cortical Neurons Display Homeostatic Plasticity. Article Snippet: Fixed cells were then permeabilized using a PBS solution containing 0.2% Triton X-100 (Merck Life Science) for 15 min and blocked with the same solution supplemented with 5% goat serum (Merck Life Science) for 20 min at room temperature. .. Cells were then incubated with primary antibodies overnight at 4 ◦C using the following dilutions: mouse anti-PAX6 (sc81649 Santa Cruz Biotechnology, 1:50), chicken anti-MAP2 (ab5392 Abcam, 1:2000), rabbit anti-β-TUBULIN III (TUJ1) (T2200 Merck Life Science, 1:2000), mouse anti-GFAP (MAB360 Merck Life Science, 1:500), rabbit anti-PSD95 (3450 Cell Signaling, Danvers, MA; USA, 1:250), mouse anti-VGLUT1 (135303 Synaptic Systems, Göttingen, Germany, 1:250), Article Title: Cognitive impairments in a Down syndrome model with abnormal hippocampal and prefrontal dynamics and cytoarchitecture. Article Snippet: They were mixed with 4X NuPAGE LDS Sample Buffer (Thermo Fisher Scientific) containing b-mercaptoethanol, denaturalized at 95 C for 5 min and resolved onto NuPAGE 4 to 12% Bis-Tris polyacrylamide gels (Thermo Fisher Scientific) using NuPAGE MOPS SDS Running Buffer (20X; Thermofisher) for mediumsize to big targeted-proteins and NuPAGE MES SDS Running Buffer (20X; Thermofisher) for small ones. .. Proteins were then transferred onto 0.2 mmnitrocellulosemembranes using Trans-Blot Turbo transfer system (Bio-Rad) at 1.3 A for 10 min. Membranes were blocked for 1 h with Intercept (PBS) Blocking Buffer and incubated overnight at 4 C with the corresponding primary antibodies ( Western Blot:Article Title: N-Methyl-D-aspartate receptor antagonist d -methadone produces rapid, mTORC1-dependent antidepressant effects Article Snippet: .. Homogenates were submitted to western blot analysis and the bands quantified as previously described [ 7 , 10 ] (primary antibodies: rabbit anti-phospho-p70S6K, rabbit anti-p70S6K, rabbit anti-phospho-p44-42 ERK, rabbit anti-p44-42 ERK, rabbit anti-phospho-4EBP1, rabbit anti-phospho-mTOR, rabbit anti-mTOR, rabbit anti-GluA1, Blocking Assay:Article Title: Cognitive impairments in a Down syndrome model with abnormal hippocampal and prefrontal dynamics and cytoarchitecture. Article Snippet: They were mixed with 4X NuPAGE LDS Sample Buffer (Thermo Fisher Scientific) containing b-mercaptoethanol, denaturalized at 95 C for 5 min and resolved onto NuPAGE 4 to 12% Bis-Tris polyacrylamide gels (Thermo Fisher Scientific) using NuPAGE MOPS SDS Running Buffer (20X; Thermofisher) for mediumsize to big targeted-proteins and NuPAGE MES SDS Running Buffer (20X; Thermofisher) for small ones. .. Proteins were then transferred onto 0.2 mmnitrocellulosemembranes using Trans-Blot Turbo transfer system (Bio-Rad) at 1.3 A for 10 min. Membranes were blocked for 1 h with Intercept (PBS) Blocking Buffer and incubated overnight at 4 C with the corresponding primary antibodies ( Immunohistochemistry:Article Title: Chronic maternal exposure to low-dose PM 2.5 impacts cognitive outcomes in a sex-dependent manner. Article Snippet: .. After deparaffinisation and rehydration through graded ethanol solutions, antigen retrieval was performed by immersing all slides in citrate antigen retrieval solution buffer (Sangon Biotech, China) (pH=6.0) at 100 ◦C for 15 min. Immunohistochemistry was carried out using the following primary antibodies: mouse anti-Tuj1 (1:500, MMS435P, Biolegend, USA), rabbit anti-MAP2 (1:500, 4542s, CST, USA), |
